Description |
Goat anti-TIRAP / Mal (Isoform b) Antibody Principle Name: TIRAP / Mal (Isoform b) Official Symbol: TIRAP All Name and Symbols: TIRAP; Mal; toll-interleukin 1 receptor (TIR) domain-containing adapter protein; adapter protein wyatt; MyD88 adapter-like protein; FLJ42305; wyatt; OTTHUMP00000179130; Toll-interleukin 1 receptor domain-containing adaptor protein; Toll-like receptor ada Accession Number(s): NP_683708.1 Peptide Sequence: EGEGERDSATVSDL, is from C Terminus Human Gene ID(s): 114609 Non-Human GeneID(s): Important Comments: This protein is expected to recognise isoform b (NP_683708.1) only. Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 128000. Western Blot: Approx 30kDa band observed in Human Liver lysates (calculated MW of 25.3kDa according to NP_683708.1). Recommended concentration: 1-3ug/ml. Reference(s): Andreakos E, Sacre SM, Smith C, Lundberg A, Kiriakidis S, Stonehouse T, Monaco C, Feldmann M, Foxwell BM. Distinct pathways of LPS-induced NF-kappa B activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of MyD88 and Mal/TIRAP. Blood. 2004 Mar 15; 103(6):2229-37..PMID: 14630816 |