Goat anti-ACADM Antibody

Goat anti-ACADM Antibody
Principle Name: ACADM
Official Symbol: ACADM
All Name and Symbols: ACADM; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; RP4-682C21.1; ACAD1; MCAD; MCADH; medium-chain acyl-CoA dehydrogenase; FLJ18227; FLJ93013; FLJ99884; medium-chain specific acyl-CoA dehydrogenase
Accession Number(s): NP_000007.1; NP_001120800.1
Peptide Sequence: RLIVAREHIDKYKN, is from C Terminus
Human Gene ID(s): 34
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). Recommended for use at 0.01-0.1ug/ml.
Immunohistochemistry: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5ug/ml.
Reference(s): Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K. Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1. Proc Natl Acad Sci U S A. 1986 Sep; 83(17):6543-7..PMID: 3462713