Description |
Goat anti-ACADM Antibody Principle Name: ACADM Official Symbol: ACADM All Name and Symbols: ACADM; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; RP4-682C21.1; ACAD1; MCAD; MCADH; medium-chain acyl-CoA dehydrogenase; FLJ18227; FLJ93013; FLJ99884; medium-chain specific acyl-CoA dehydrogenase Accession Number(s): NP_000007.1; NP_001120800.1 Peptide Sequence: RLIVAREHIDKYKN, is from C Terminus Human Gene ID(s): 34 Non-Human GeneID(s): Important Comments: This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1). Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). Recommended for use at 0.01-0.1ug/ml. Immunohistochemistry: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5ug/ml. Reference(s): Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K. Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1. Proc Natl Acad Sci U S A. 1986 Sep; 83(17):6543-7..PMID: 3462713 |