Description |
Goat anti-Dispatched homolog 1 Antibody Principle Name: Dispatched homolog 1 Official Symbol: DISP1 All Name and Symbols: DISP1; DISPA; MGC13130; MGC16796; DKFZP434I0428; dispatched A; DISP1; dispatched homolog 1; dispatched homolog 1 (Drosophila); FLJ43740; MGC104180 Accession Number(s): NP_116279.2 Peptide Sequence: SELSGESLLIKTL, is from C Terminus Human Gene ID(s): 84976 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IF Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1ug/ml. Reference(s): Burke R, Nellen D, Bellotto M, Hafen E, Senti KA, Dickson BJ, Basler K. Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells. Cell. 1999 Dec 23; 99(7):803-15..PMID: 10619433 |