Goat anti-FOXD1 / FREAC4 / FKHL8 Antibody

Goat anti-FOXD1 / FREAC4 / FKHL8 Antibody
Principle Name: FOXD1 / FREAC4 / FKHL8
Official Symbol: FOXD1
All Name and Symbols: FOXD1; forkhead box D1; FKHL8; FREAC4; forkhead (Drosophila)-like 8; forkhead-related activator 4; Forkhead, drosophila, homolog-like 8; forkhead-like 8
Accession Number(s): NP_004463.1
Peptide Sequence: SSVENFTARISNC, is from C Terminus
Human Gene ID(s): 2297
Non-Human GeneID(s): 15229 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: No signal obtained yet but low background observed in Human Kidney extracts at up to 1ug/ml.
Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 2.5ug/ml.
Reference(s): Ernstsson S, Pierrou S, Hulander M, Cederberg A, Hellqvist M, Carlsson P, Enerback S. Characterization of the human forkhead gene FREAC-4. Evidence for regulation by Wilms' tumor suppressor gene (WT-1) and p53. J Biol Chem. 1996 Aug 30; 271(35):21094-9..PMID: 8702877