Goat anti-RIG / DKK3 Antibody

Goat anti-RIG / DKK3 Antibody
Principle Name: RIG / DKK3
Official Symbol: DKK3
All Name and Symbols: DKK3; dickkopf homolog 3 (Xenopus laevis); REIC; RIG; RIG-like 5-6; RIG-like 7-1; dickkopf 3; dickkopf-3; dickkopf-related protein 3; dkk-3; hDkk-3; regulated in glioma
Accession Number(s): AAC35996.1
Peptide Sequence: TLSVVGPHPKPCIL, is from C Terminus
Human Gene ID(s): 10530
Non-Human GeneID(s):
Important Comments: NCBI has removed RIG (GeneID 10530) and has it replaced by DKK3 (GeneID 27122). The AAC35996 protein is no longer represented by an NP RefSeq code.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: No signal obtained yet but low background observed in Human Heart lysate at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry: In paraffin embedded Human Uterus shows membranous staining in the endometrial glands. Recommended concentration, 2-6ug/ml.
Reference(s): Ligon AH, Pershouse MA, Jasser SA, Yung WK, Steck PA. Identification of a novel gene product, RIG, that is down-regulated in human glioblastoma. Oncogene. 1997 Mar 6; 14(9):1075-81..PMID: 9070656