Description |
Goat anti-PNKP / PNK Antibody Principle Name: PNKP / PNK Official Symbol: PNKP All Name and Symbols: PNKP; polynucleotide kinase 3'-phosphatase; PNK; polynucleotide kinase 3-prime-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); Accession Number(s): NP_009185.2 Peptide Sequence: EPRLGRLYCQFSEG, is from C Terminus Human Gene ID(s): 11284 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 16000. Western Blot: Sucessfully used to detect recombinant human PNKP (1/1000 dilution). Reference(s): Jilani A, Ramotar D, Slack C, Ong C, Yang XM, Scherer SW, Lasko DD. Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage. J Biol Chem. 1999 Aug 20; 274(34):24176-86..PMID: 10446192 |