SensoLyte 520 Meprin Beta Activity Assay Kit *Fluorimetric*

Meprin beta, a type I transmembrane metalloprotease, belongs to the astacin family of zinc endopeptidases and the metzincin superfamily. Protein expression has been identified in multiple tissues and cell types such as kidney, intestine, skin, leukocytes and several cancer cells. Though the biological function of Meprin beta is not fully understood, it was found that Meprin beta plays various roles in inflammatory response, collagen assembly, cancer progression and beta-amyloid protein cleavage. More growing evidence has indicated that Meprin beta is a good therapeutic target in regulating angiogenesis, cancer, inflammation, fibrosis, and neurodegenerative diseases. The SensoLyte 520 Meprin beta Activity Assay Kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The unique FRET substrate was derived from APP sequence designed to reduce the cross reactivity with Meprin alpha, ADAM10, alpha-secretase, BACE-2, IDE and ECEs, Neprilysin and TACE. Active Meprin beta cleaves the FRET substrate resulting in an increase of 5-FAM fluorescence, that can be monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.048 ng/mL active Meprin beta. The kit contains: - 5-FAM/QXL 520 Meprin beta substrate, Ex/Em=490/520 nm upon cleavage - Assay buffer - Recombinant Human Meprin beta - Meprin beta activation buffer - Meprin beta stop solution - Inhibitor - Fluorescence reference standard for calibration - A detailed protocol Kit size: 100 assays (96-well plate) Figure 1. Inhibition of Meprin beta activity by Actinonin as measured with SensoLyte 520 Meprin beta Activity Assay Kit.

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