SensoLyte® 520 Cathepsin G Assay Kit Fluorimetric - 1 kit

Assay Kits

Dry Ice

Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs. The SensoLyte 520 Cathepsin G Activity Assay Kit uses a HiLyte Fluor 488/QXL 520 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor 488 is quenched by QXL 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte Fluor 488/QXL 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The fluorescent signal from HiLyte Fluor 488 is stable at low pH, which is the optimal pH for cathepsin activity. The kit can be used to detect the activity of Cathepsin G enzyme in biological samples and purified enzyme preparations. The kit contains: - HiLyte Fluor 488/QXL520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) - Assay buffer - Human Cathepsin G - Inhibitor - Fluorescence reference standard for calibration - A detailed protocol Kit size: 100 assays (96-well plate)

Viral & bacterial Proteases / 0

520

Fluorescent

41105331