SensoLyte® 520 Cathepsin D Assay Kit *Fluorimetric* - 1 kit

Assay Kits

Dry Ice

Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease. The SensoLyte 520 Cathepsin D Activity Assay Kit uses a QXL 520/HiLyte Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor 488 is quenched by QXL 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations. The kit contains: - HiLyte Fluor 488/QXL520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) - Assay buffer - Bovine spleen Cathepsin D - Inhibitor - Fluorescence reference standard for calibration - A detailed protocol Kit size: 100 assays (96-well plate)

Cancer & Apoptosis; Neuroscience / 0

520

Fluorescent

Research use