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SensoLyte® ONPG ß-Galactosidase Assay Kit Colorimetric - 1 kit

SensoLyte® ONPG ß-Galactosidase Assay Kit Colorimetric - 1 kit

Item no.	AS-72134
Manufacturer	AnaSpec
Amount	1 kit
Category	Cell Biology Cell Status Senescence Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	ß-Galactosidase,Beta-Galactosidase,GLB1 ELNR1
Shipping Condition	Dry ice
Available
Manufacturer - Applications	Enzyme activity
Manufacturer - Category	Assays Kits Group 4 / Assays Kits Group 4 / Assay Kits
Manufacturer - Targets	Beta-Galactosidase
Shipping Temperature	4 °C
Storage Conditions	Store all components at -20°C. Components C and D can be stored at room temperature for convenience. Protect component A from light.
Manufacturer - Research Area	Cell Biology & Protein Analysis / Protein Assay Kits
Description	?-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte® ONPG ?-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl ?-D-galactopyranoside (ONPG) to determine the activity of ?-galactosidase in transfected cells or tissues. In the presence of ?-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to ?-galactosidase activity.
Kits Components	Component A: Substrate solution: 50 mL Component B: ß -galactosidase enzyme: 0.1 mg/mL, 20 µL Component C: Lysis buffe:r 100 mL Component D: Stop solution: 30 mL Component E: DTT: 1 M, 5 mL
Absorbtion (nm)	420
Detection Method	Colorimetric
Detection Limit	1 ng/ml
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	41105333
GTIN	5400535131395

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-72134-safety-datasheet.pdf