SensoLyte® ONPG β-Galactosidase Assay Kit *Colorimetric* - 1 kit

Assay Kits

4 °C

beta-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte ONPG beta-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl beta-D-galactopyranoside (ONPG) to determine the activity of beta-galactosidase in transfected cells or tissues. In the presence of beta-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to beta-galactosidase activity. The kit contains: b-galactosidase substrate, beta-galactosidase standard, reaction buffer, stop buffer and optimized assay protocol. Assays are performed in a convenient 96-well format. Kit size: 500 assays

Cell Biology & Protein Analysis / 0

5400535131395

1 ng/ml

41105333