Item no. |
ARP-28-10309-100 |
Manufacturer |
American Research Products
|
Amount |
100 ug |
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Description |
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces transformation anchorage independence in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. Recombinant human TGF-alpha is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds. |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
Sacroma growth factor, TGF-type I, ETGF |
Purity |
0, 98 |
Source |
E. Coli |
Interest Fields |
Angiogenesis/Cardiovascular, Cancer, Inflammation, Wound Healing |
Amino Acid Sequence |
VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA |
Endotoxin Level |
Endotoxin level is <, 0.1 ng/ug of protein (<, 1EU/ug). |
Biological Activity |
The ED 50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is a‰¤ 0.2 ng/ml, corresponding to a specific activity of a‰¥ 5 x 10^6 units/mg. |
Protein Content |
Verified by UV Spectroscopy and/or SDS-PAGE gel. |
Authenticity |
Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.