Manufacturer |
American Research Products
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Amount |
1 mg |
Item no. |
ARP-28-10231-1 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Description |
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens. Recombinant human MMP-1 is a 42.7 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain which is involved in substrate specificity and in binding TIMP-1. |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
Fibroblast collagenase, Interstitial collagenase |
Purity |
0, 9 |
Source |
E. Coli |
Interest Fields |
Angiogenesis/Cardiovascular, Bone, Skeletal, Cartilage, Cancer, Wound Healing |
Amino Acid Sequence |
MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEK AFQLWSNVTP LTFTKVSEGQ ADIMISFVRG DHRDNSPFDG PGGNLAHAFQ PGPGIGGDAH FDEDERWTNN FREYNLHRVA AHELGHSLGL SHSTDIGALM YPSYTFSGDV QLAQDDIDGI QAIYGRSQNP VQPIGPQTPK ACDSKLTFDA ITTIRGEVMFF KDRFYMRTNP FYPEVELNFI SVFWPQLPNG LEAAYEFADR DEVRFFKGNK YWAVQGQNVL HGYPKDIYSS FGFPRTVKHI DAALSEENTG KTYFFVANKY WRYDEYKRSM DPGYPKMIAH DFPGIGHKVD AVFMKDGFFY FFHGTRQYKF DPKTKRILTL QKANSWFNCR KN |
Endotoxin Level |
Endotoxin level is <, 0.1 ng/ug of protein (<, 1EU/ug). |
Biological Activity |
MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 ug/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes |
Protein Content |
Verified by UV Spectroscopy and/or SDS-PAGE gel. |
Authenticity |
Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.