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UBA3, 1-463aa, Human, His tag, E.coli

UBA3, 1-463aa, Human, His tag, E.coli

Manufacturer	American Research Products
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Specific against	other
Amount	20 ug
Item no.	ARP-01-P1425-20
eClass 6.1	34160400
eClass 9.0	42020190
Available
Description	UBA3, also known as NEDD8-activating enzyme E1 catalytic subunit, is the catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Recombinant human UBA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Form	Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
Storage	Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms	NEDD8-activating enzyme E1 catalytic subunit, UBE1C
Concentration	1 mg/ml (determined by Bradford assay)
NCBI	AAH22853
References	Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042 Bohnsack R.N., et al. (2003) J. Biol. Chem. 278:26823-26830
Antigen Recognized in Species	Human
Purity	>, 90% by SDS - PAGE
Molecular Weight	54.4 kDa (487aa)
Amino Acid Sequence	MGSSHHHHHH SSGLVPRGSH MGSHMADGEE PERKRRRIEE LLAEKMAVDG GCGDTGDWEG RWNHVKKFLE RSGPFTHPDF EPSTESLQFL LDTCKVLVIG AGGLGCELLK NLALSGFRQI HVIDMDTIDV SNLNRQFLFR PKDIGRPKAE VAAEFLNDRV PNCNVVPHFN KIQDFNDTFY RQFHIIVCGL DSIIARRWIN GMLISLLNYE DGVLDPSSIV PLIDGGTEGF KGNARVILPG MTACIECTLE LYPPQVNFPM CTIASMPRLP EHCIEYVRML QWPKEQPFGE GVPLDGDDPE HIQWIFQKSL ERASQYNIRG VTYRLTQGVV KRIIPAVAST NAVIAAVCAT EVFKIATSAY IPLNNYLVFN DVDGLYTYTF EAERKENCPA CSQLPQNIQF SPSAKLQEVL DYLTNSASLQ MKSPAITATL EGKNRTLYLQ SVTSIEERTR PNLSKTLKEL GLVDGQELAV ADVTTPQTVL FKLHFTS
Gene Category	Ubiquitination

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 20 ug

available

Delivery expected until 7/19/2024