Manufacturer |
American Research Products
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Amount |
100 ug |
Item no. |
ARP-01-2113-1-100 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Description |
Carbonic anhydrase 1(CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O <, ->, HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
Form |
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
CA1, CA-I, CAB, JW0122, CA 1, CA I, CAI, CAN, Car 1, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, Carbonic dehydratase, ECK0125, yadF, |
Concentration |
1 mg/ml (determined by Bradford assay) |
NCBI |
NP_001122301 |
References |
- Nogradi A., et al. (1998). Am J Pathol. 153:1-4
- Ferry JG., et al. (1999). Proc Natl Acad Sci U S A. 96(26):15184-9.
|
Antigen Recognized in Species |
Human |
Purity |
>, 95% by SDS - PAGE |
Molecular Weight |
31.0 kDa (281aa), confirmed by MALDI-TOF. |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F |
Gene Category |
Metabolism |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.