Item no. |
ARP-01-1754-1-0.5 |
Manufacturer |
American Research Products
|
Amount |
0.5 mg |
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Description |
LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity. Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques |
Form |
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1 |
Concentration |
0.5 mg/ml (determined by Bradford assay) |
NCBI |
NP_032892 |
References |
- Schaloske R.H., et al. (2006) Biochim Biophys Acta. 1761:1246-1259.
- Hiraoka M., et al. (2002) J. Biol. Chem 277:10090-10099.
|
Antigen Recognized in Species |
mouse |
Purity |
>, 90% by SDS - PAGE |
Molecular Weight |
26.8 kDa (250aa), confirmed by MALDI-TOF |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID |
Gene Category |
Metabolism |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.