Item no. |
ARP-10-P1952 |
Manufacturer |
American Research Products
|
Amount |
100 ug |
Category |
|
Type |
Antibody Polyclonal |
Applications |
ICC |
Specific against |
other |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Description |
Rabbit IgG polyclonal antibody for NADPH--cytochrome P450 reductase (POR) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat. |
Background |
POR (Cytochrome P450 Oxidoreductase), also known as Cytochrome P450 Reductase, is a membrane-bound enzyme required for electron transfer to cytochrome P450 in the endoplasmic reticulumof the eukaryotic cell from a FAD- and FMN-containing enzyme NADPH: cytochrome P450 reductase. By Southern blot analysis of DNA isolated from a panel of 8 independent human-rodent somatic cell hybrids, Shephard et al. (1989) determined that cytochrome P450 reductase is encoded by a single gene located on 7pter-q22. By in situ hybridization to metaphase chromosomes, they refined the localization to 7q11.2. Cytochrome P450 oxidoreductase is a flavoprotein that donates electrons to all microsomal P450 enzymes, including the steroidogenic enzymes P450c17 (CYP17A1), P450c21 (CYP21A2), and CYP51A1 (Miller, 1986). |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminal of human POR, different from the related rat and mouse sequences by one amino acid. |
Form |
Lyophilized |
Specificity |
Human, Mouse, Rat. No cross reactivity with other proteins. |
Storage |
2-8C. For long term -20C after reconstitution. |
References |
- 1. Adachi, M., Asakura, Y., Matsuo, M., Yamamoto, T., Hanaki, K., Arlt, W. POR R457H is a global founder mutation causing Antley-Bixler syndrome with autosomal recessive trait. (Letter) Am. J. Med. Genet. 140A: 633-635, 2006.
- 2. Miller, W. L. Congenital adrenal hyperplasia. (Letter) New Eng. J. Med. 314: 1321-1322, 1986.
- 3. Shephard, E. A., Phillips, I. R., Santisteban, I., West, L. F., Palmer, C. N., Ashworth, A., Povey, S. Isolation of a human cytochrome P-450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2. Ann. Hum. Genet. 53: 291-301, 1989.
|
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Purification |
Immunogen affinity purified. |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.