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Anti-CRKL

Anti-CRKL

Item no.	ARP-10-P1808
Manufacturer	American Research Products
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Polyclonal
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Description	Rabbit IgG polyclonal antibody for Crk-like protein (CRKL) detection. Tested with WB in Human, Mouse, Rat.
Background	CRKL(v-crk sarcoma virus CT10 oncogene homolog (avian)-like) also called ONCOGENEA CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. WhileA CRKLA is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization, A theA CRKLA gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al. (1996)A showed thatA CRKLA becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also foundA CRKLA to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKLA is a tyrosine kinase and an oncogene. CrklA was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminal of human CRKL, different from the related mouse and rat sequences by one amino acid.
Form	Lyophilized
Specificity	Human, Mouse, Rat. No cross reactivity with other proteins.
Storage	2-8C. For long term -20C after reconstitution.
References	1. Guris, D. L., Duester, G., Papaioannou, V. E., Imamoto, A.A Dose-dependent interaction of Tbx1 andA CrklA and locally aberrant RA signaling in a model of del22q11 syndrome.A Dev. Cell 10: 81-92, 2006. 2. Moon, A. M., Guris, D. L., Seo, J., Li, L., Hammond, J., Talbot, A., Imamoto, A.A CrklA deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes.A Dev. Cell 10: 71-80, 2006. 3. Senechal, K., Halpern, J., Sawyers, C. L.A TheA CRKLA adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene.A J. Biol. Chem. 38: 23255-23261, 1996.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Purification	Immunogen affinity purified.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 8/23/2024