Item no. |
ARP-10-P1044 |
Manufacturer |
American Research Products
|
Amount |
100 ug |
Category |
|
Type |
Antibody Polyclonal |
Specific against |
other |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Description |
Rabbit IgG polyclonal antibody for Insulin-like 3 (INSL3) detection. Tested with WB, IHC-P in Human, Mouse, Rat. |
Background |
Insulin-like 3 (INSL3), a member of the insulin-like hormone superfamily, is specifically expressed in Leydig cells of the fetal and postnatal testis and in theca cells of the postnatal ovary. It is synthesized as a 131-amino acid preproprotein, which contains a 24-amino acid signal peptide. The human INSL3 gene is assigned to bands p13.2-p12 of the short arm of chromosome 19 with the similar organization to that of insulin and relaxin. INSL3 induces gubernaculum development in an androgen-independent way, while androgen-mediated regression of the CSL occurs independently from Insl3. Moreover, INSL3 is a ligand for LGR8 and INSL3-LGR8 mutations are believed to be associated with human cryptorchidism. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminal of human INSL3, different from the related rat and mouse sequences by three amino acids. |
Form |
Lyophilized |
Specificity |
Human, Mouse, Rat. No cross reactivity with other proteins. |
Storage |
2-8C. For long term -20C after reconstitution. |
Synonyms |
Leydig insulin-like peptide |
References |
- 1. Adham IM, Burkhardt E, Benahmed M, Engel W.Cloning of a cDNA for a novel insulin-like peptide of the testicular Leydig cells. J Biol Chem. 1993 Dec 15, 268(35):26668-72.
- 2. Burkhardt E, Adham IM, Brosig B, Gastmann A, Mattei MG, Engel W.Structural organization of the porcine and human genes coding for a Leydig cell-specific insulin-like peptide (LEY I-L) and chromosomal localization of the human gene (INSL3). Genomics. 1994 Mar 1, 20(1):13-9.
- 3. Zimmermann S, Steding G, Emmen JM, Brinkmann AO, Nayernia K, Holstein AF, Engel W, Adham IM.Targeted disruption of the Insl3 gene causes bilateral cryptorchidism. Mol Endocrinol. 1999 May, 13(5):681-91.
- 4. Foresta C, Ferlin A.Role of INSL3 and LGR8 in cryptorchidism and testicular functions. Reprod Biomed Online. 2004 Sep, 9(3):294-8.
|
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Purification |
Immunogen affinity purified. |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.