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TriMethyl-Histone H3-K27 Mouse mAb

TriMethyl-Histone H3-K27 Mouse mAb

Item no.	A16199-100ul
Manufacturer	Abclonal
Amount	100ul
Category	Antibodies Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies CHiP
Type	Antibody Monoclonal
Applications	WB, IF, ICC, ELISA, IHC-P, CHIP, Dot
Specific against	Human
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
Purity	Affinity purification
NCBI	H3-4
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Available
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Route	Synthetic peptide
Manufacturers Category	Methylated Antibodies
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Storage	Store at -20℃. Avoid freeze / thaw cycles.\|Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Recommended Dilution	DB, 1:500 - 1:2000\|WB, 1:500 - 1:1000\|IHC-P, 1:50 - 1:200\|IF/ICC, 1:50 - 1:200\|ChIP, 1:20 - 1:100\|CUT&Tag, 10⁵ cells /1 μg
Protein Size	16kDa
Manufacturers Research Area	Epigenetics Nuclear Signaling, Protein phosphorylation, Signal Transduction, MAPK-Erk Signaling Pathway
Gene Symbol	H3-4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ul

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