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TraKine F-actin Staining Kit (Green Fluorescence)

TraKine F-actin Staining Kit (Green Fluorescence)

Item no.	ABK-KTC4008-300T
Manufacturer	Abbkine Scientific
Amount	300T
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Applications notes	Abbkine TraKine F-actin Staining Kit (Green Fluorescence) uses a green fluorescent (Ex/Em=490/515nm) phalloidin conjugate selectively bound to F-actins, it is much higher photostability than the other fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels.
Kit components	Phalloidin (Green Fluorescence) (200x) Assay Buffer (5x)
Features & Benefits	Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Proprietary Phalloidin (Green Fluorescence) (Ex/Em = 490/515 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.
Usage notes	As the optimal staining conditions may vary among different cell types, we recommend that a suitable concentration of Phalloidin (Green Fluorescence) be determined.
Storage instructions	Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 300T

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Delivery expected until 10/18/2024

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