Comparison

Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit

Item no. ABK-KTE100656-48T
Manufacturer Abbkine Scientific
Amount 48T
Category
Type Elisa-Kit
Applications ELISA
Specific against Rat
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit employs a two-site sandwich ELISA to quantitate MAVS in samples. An antibody specific for MAVS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAVS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAVS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAVS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Rat Mitochondrial antiviral-signaling protein microplate
Rat Mitochondrial antiviral-signaling protein standard
Rat Mitochondrial antiviral-signaling protein detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAVS. No significant cross-reactivity or interference between Rat MAVS and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK) and IKKB (IKBKB), which activate NFKB , or TBK1 and IKKE (IKBKE), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005).Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
Gene ID
311430
Alternative
MAVS; CARDIF; DKFZp547C224; DKFZp666M015; FLJ27482; FLJ35386; FLJ38051; FLJ41962; IPS-1; IPS1; KIAA1271; MGC3260; VISA; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; IFN-B promoter
Accession
Q66HG9

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 48T
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close