Rat Ras-related C3 Botulinum Toxin Substrate 1 (RAC1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rat Ras-related C3 Botulinum Toxin Substrate 1 microplate
Rat Ras-related C3 Botulinum Toxin Substrate 1 standard
Rat Ras-related C3 Botulinum Toxin Substrate 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. This mutant did bind to POR1 and it induced membrane ruffling and transformation. A second RAC mutant, which bound PAK but not POR1, induced JNK activation but was defective in inducing membrane ruffling and transformation. The authors concluded that the effects of RAC on the JNK cascade and on actin polymerization and cell proliferation are mediated by distinct effector functions that diverge at the level of RAC itself.

RAC1; MGC111543; MIG5; TC-25; p21-Rac1; migration-inducing gene 5; ras-related C3 botulinum toxin substrate 1; rho family; small GTP binding protein Rac1