Mouse Interleukin-7 (IL-7) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Interleukin-7 microplate
Mouse Interleukin-7 standard
Mouse Interleukin-7 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Interleukin 7 is a type I short-chain cytokine belonging to the hematopoietin family. Mouse IL-7 is an approximately 25 kDa monomeric glycoprotein that is synthesized as a 154 amino acid (aa) residue precursor with a 25 aa signal sequence and a 129 aa mature segment. Mature mouse IL-7 has six cysteine residues that form three intrachain disulfide bonds, only one of which is required for bioactivity. Although multiple alternatively spliced isoforms of human IL-7 that differ in their receptor-binding affinities exist, alternatively spliced isoforms of mouse IL-7 have not been reported. Cells known to express IL-7 include Class II MHC positive thymic cortical epithelial cells, follicular dendritic cells, intestinal epithelium, monocytes and keratinocytes, endothelial cells, vascular smooth muscle cells and fibroblasts, and bone marrow stromal cells.

IL7; IL-7; IL7 nirs variant 6