Mouse Testisin (PRSS21) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Testisin microplate
Mouse Testisin standard
Mouse Testisin detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

PRSS21 encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. It is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and it may be involved in progression of testicular tumors of germ cell origin. The deduced 314-amino acid ESP1 precursor protein has a calculated molecular mass of 33.1 kD, and the 273-amino acid active form has a calculated molecular mass of 30.6 kD.
ESP1 contains an N-terminal signal peptide, a propeptide, an active site catalytic triad of conserved his, asp, and ser residues, and a hydrophobic C terminus, which suggests that ESP1 is a membrane-type serine protease.

PRSS21; ESP-1; ESP1; TEST1; TESTISIN; serine protease from eosinophils; testisin