Item no. |
ABK-KTE70468-96T |
Manufacturer |
Abbkine Scientific
|
Amount |
96T |
Category |
|
Type |
Elisa-Kit |
Applications |
ELISA |
Specific against |
Mouse |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Available |
|
Applications notes |
This Mouse Succinate dehydrogenase cytochrome b560 subunit, mitochondrial (SDHC) ELISA Kit employs a two-site sandwich ELISA to quantitate SDHC in samples. An antibody specific for SDHC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDHC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHC bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Mouse Succinate dehydrogenase cytochrome b560 subunit, mitochondrial microplate Mouse Succinate dehydrogenase cytochrome b560 subunit, mitochondrial standard Mouse Succinate dehydrogenase cytochrome b560 subunit, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Mouse Succinate dehydrogenase cytochrome b560 subunit, mitochondrial (SDHC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SDHC. No significant cross-reactivity or interference between Mouse SDHC and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
SDHC encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. Several related pseudogenes are located in different genomic regions. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants encoding different isoforms have been described. |
Gene ID |
66052 |
Alternative |
SDHC; CYB560; CYBL; PGL3; QPS1; SDH3; cytochrome B large subunit of complex; integral membrane protein CII-3; integral membrane protein CII-3b; succinate dehydrogenase complex; subunit C; succinate dehydro |
Accession |
Q9CZB0 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.