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Mouse Tankyrase-2 (TNKS2) ELISA Kit

Mouse Tankyrase-2 (TNKS2) ELISA Kit

Manufacturer	Abbkine Scientific
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Mouse
Applications	ELISA
Amount	48T
Item no.	ABK-KTE70105-48T
eClass 6.1	32160605
eClass 9.0	32160605
Available
Applications notes	This Mouse Tankyrase-2 (TNKS2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNKS2 in samples. An antibody specific for TNKS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNKS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
Sample type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	Mouse Tankyrase-2 microplate Mouse Tankyrase-2 standard Mouse Tankyrase-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features & Benefits	Mouse Tankyrase-2 (TNKS2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNKS2. No significant cross-reactivity or interference between Mouse TNKS2 and analogues was observed.
Usage notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The poly(ADP-ribose) polymerase (PARP) tankyrase-1 contains an ankyrin-repeat domain that binds to various partners, including the telomeric protein TRF1 (telomere-repeat-binding factor 1) and the vesicular protein IRAP (insulin-responsive aminopeptidase). TRF1 binding recruits tankyrase-1 to telomeres and allows its PARP activity to regulate telomere homoeostasis. By contrast, IRAP binding and the Golgi co-localization of tankyrase-1 with IRAP might allow tankyrase-1 to affect the targeting ofIRAP-containing vesicles. A closely related protein, tankyrase-2, has also been implicated in vesicular targeting. Unlike tankyrase-1, tankyrase-2 has not been shown to have PARP activity. In addition, it has not been implicated in telomere homoeostasis, because it did not interact with TRF1 in previous studies.
Gene ID	74493
Alternative	TNKS2; PARP-5b; PARP-5c; PARP5B; PARP5C; TANK2; TNKL; tankyrase 2
Accession	Q3UES3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48T

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Delivery expected until 8/16/2024

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