Item no. |
ABK-KTE62666-96T |
Manufacturer |
Abbkine Scientific
|
Amount |
96T |
Category |
|
Type |
Elisa-Kit |
Applications |
ELISA |
Specific against |
Human |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Available |
|
Applications notes |
This Human Angiotensin I (ANG-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ANG-1 in samples. An antibody specific for ANG-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANG-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Human Angiotensin I microplate Human Angiotensin I standard Human Angiotensin I detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Human Angiotensin I (ANG-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ANG-1. No significant cross-reactivity or interference between Human ANG-1 and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Angiotensin I is formed by the action of renin on angiotensinogen. Renin is produced in the kidneys in response to both decreased intra-renal blood pressure at the juxtaglomerular cells, or decreased delivery of Na+ and Cl- to the macula densa. If more Na+ is sensed, renin release is decreased.Renin cleaves the peptide bond between the leucine (Leu) and valine (Val) residues on angiotensinogen, creating the ten amino acid peptide (des-Asp) angiotensin I .Angiotensin I appears to have no biological activity and exists solely as a precursor to angiotensin 2.Angiotensin I is converted to angiotensin II through removal of two terminal residues by the enzyme Angiotensin-converting enzyme (ACE, orkinase), which is found predominantly in the capillaries of the lung. ACE is actually found all over the body, but has its highest density in the lung due to the high density of capillary beds there. Angiotensin II acts as an endocrine, autocrine/paracrine, and intracrine hormone. |
Gene ID |
1636 |
Alternative |
ANG-1; Angiotensin I ; AngI |
Accession |
P12821 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.