Human Protein FAM3B (FAM3B) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Protein FAM3B microplate
Human Protein FAM3B standard
Human Protein FAM3B detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

The authors predicted that FAM3B forms a 4-helix bundle, and 3-dimensional modeling indicated that 4 cysteines could form 2 disulfide bonds linking helices 1 and 4 and helices 2 and 3. The human and mouse FAM3B proteins share 78% amino acid identity. Northern blot analysis detected FAM3B expression at a high level in pancreas and at lower levels in small intestine and prostate. Immunohistochemical analysis of mouse tissues detected prominent Fam3b staining in the endocrine pancreas, specifically in the islets of Langerhans. Staining was also found in round spermatids within the seminiferous tubules, in the Purkinje cell layer of the cerebellum, and in nerve cell bodies of numerous brainstem nuclei. Transfected Chinese hamster ovary cells secreted Fam3b to the culture medium.

FAM3B; 2-21; C21orf11; C21orf76; ORF9; PANDER; PRED44; D21M16SJHU19e; cytokine-like protein 2-21; pancreatic derived factor