Human Plasmalemma vesicle-associated protein (PLVAP) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Plasmalemma vesicle-associated protein microplate
Human Plasmalemma vesicle-associated protein standard
Human Plasmalemma vesicle-associated protein detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

PLVAP is a type II integral membrane protein with a short N-terminal cytoplasmic tail, a single-span transmembrane domain, and a large C-terminal extracellular domain consisting mostly of alpha helices. It also contains 2 coiled-coil domains predicted to contribute to dimer formation. The extracellular domain has 4 N-glycosylation sites, a proline-rich region, and regular spacing of cysteine residues. The deduced 439-amino acid mouse Plvap protein contains a consensus casein kinase II phosphorylation site that is not found in the human protein. Rat Plvap contains 2 casein kinase II phosphorylation sites. Northern blot analysis revealed a single PLVAP transcript of about 2.4 kb in pancreas, kidney, placenta, muscle, lung, heart, and liver. No signal was detected in brain or testis.

PLVAP; FELS; PV-1; PV1; gp68; fenestrated-endothelial linked structure protein; PV-1 protein