Human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit

This Human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP7B1 in samples. An antibody specific for CYP7B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP7B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Cell culture supernatants#Serum#Plasma#Other biological fluids

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYP7B1. No significant cross-reactivity or interference between Human CYP7B1 and analogues was observed.

The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

9420

O75881