Human Keratin, type II cytoskeletal 6A (KRT6A) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Keratin, type II cytoskeletal 6A microplate
Human Keratin, type II cytoskeletal 6A standard
Human Keratin, type II cytoskeletal 6A detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Keratin 6A is a type II cytokeratin, one of a number of isoforms of keratin 6 encoded by separate genes located within the type II keratin gene cluster on human chromosome 12q. It is found with keratin 16 and/or keratin 17 in the palm and sole epidermis, the epithelial cells of the nail bed, the filiform papillae of the tongue, the epithelial lining of oral mucosa and esophagus, as well as the hair follicles. This keratin 6 isoform is thought to be the most abundant of the K6 isoforms.
The KRT6A gene consists of 9 exons separated by 8 introns and is located in the type II keratin gene cluster on human chromosome 12q. Keratin 6B and keratin 6C are encoded by the neighbouring genes, which are identical in intron-exon organization to KRT6A and are more than 99% identical in their DNA coding sequences.

KRT6A; CK6A; CK6C; CK6D; K6A; K6C; K6D; KRT6C; KRT6D; 56 cytoskeletal type keratin; K6D keratin; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; keratin 6C; keratin; epidermal type; K6A; keratin; epidermal