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Human Laminin subunit alpha-3 (LAMA3) ELISA Kit

Human Laminin subunit alpha-3 (LAMA3) ELISA Kit

Manufacturer	Abbkine Scientific
Category	Cell Biology ELISA Cell Stainings ECM ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human
Applications	ELISA
Amount	96T
Item no.	ABK-KTE61862-96T
eClass 6.1	32160605
eClass 9.0	32160605
Available
Applications notes	This Human Laminin subunit alpha-3 (LAMA3) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMA3 in samples. An antibody specific for LAMA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
Sample type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	Human Laminin subunit alpha-3 microplate Human Laminin subunit alpha-3 standard Human Laminin subunit alpha-3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features & Benefits	Human Laminin subunit alpha-3 (LAMA3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LAMA3. No significant cross-reactivity or interference between Human LAMA3 and analogues was observed.
Usage notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin subunit alpha-3 is the alpha-3 chain of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified.Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Gene ID	3909
Alternative	LAMA3; BM600; E170; LAMNA; LOCS; lama3a; BM600 150kD subunit; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; kalinin 165kD subunit; laminin alpha 3 subunit; laminin; alpha 3 (nicein (150kD); kalinin (165
Accession	Q16787

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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