Comparison

Human Myelin transcription factor 1-like protein (MYT1L) ELISA Kit

Item no. ABK-KTE61401-96Tx50
Manufacturer Abbkine Scientific
Amount 96Tx50
Category
Type Elisa-Kit
Applications ELISA
Specific against Human
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Human Myelin transcription factor 1-like protein (MYT1L) ELISA Kit employs a two-site sandwich ELISA to quantitate MYT1L in samples. An antibody specific for MYT1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYT1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYT1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYT1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Myelin transcription factor 1-like protein microplate
Human Myelin transcription factor 1-like protein standard
Human Myelin transcription factor 1-like protein detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Myelin transcription factor 1-like protein (MYT1L) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYT1L. No significant cross-reactivity or interference between Human MYT1L and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
MYT1L encodes a member of the zinc finger superfamily of transcription factors. T Myelin transcription factor 1-like protein belongs to a novel class of cystein-cystein-histidine-cystein zinc finger proteins that function in the developing mammalian central nervous system. Forced expression of this gene in combination with the basic helix-loop-helix transcription factor NeuroD1 and the transcription factors POU class 3 homeobox 2 and achaete-scute family basic helix-loop-helix transcription factor 1 can convert fetal and postnatal human fibroblasts into induced neuronal cells, which are able to generate action potentials. Mutations in MYT1L have been associated with autosomal mental retardation-39 and autism spectrum disorder. Alternative splicing results in multiple variants.
Gene ID
23040
Alternative
MYT1L; NZF1; neural zinc finger transcription factor 1
Accession
Q9UL68

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96Tx50
Available: In stock
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