Comparison

Chicken Matrix Gla protein (MGP) ELISA Kit

Item no. ABK-KTE30110-96Tx5
Manufacturer Abbkine Scientific
Amount 96Tx5
Category
Type Elisa-Kit
Applications ELISA
Specific against Gallus
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Chicken Matrix Gla protein (MGP) ELISA Kit employs a two-site sandwich ELISA to quantitate MGP in samples. An antibody specific for MGP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Chicken Matrix Gla protein microplate
Chicken Matrix Gla protein standard
Chicken Matrix Gla protein detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Chicken Matrix Gla protein (MGP) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken MGP. No significant cross-reactivity or interference between Chicken MGP and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Matrix gla protein (MGP) is member of a family of vitamin-K2 dependent, Gla-containing proteins. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. MGP is found in number body tissues in mammals, birds, and fish. Its mRNA is present in bone, cartilage, heart, and kidney. It is present in bone together with the related vitamin K2-dependent protein osteocalcin. In bone, its production is increased by vitamin D.
Gene ID
395912
Alternative
MGP; GIG36; MGLAP; NTI;
Accession
O42413

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96Tx5
Available: In stock
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