Fish Leukotriene B4 (LT-B4) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Fish Leukotriene B4 microplate
Fish Leukotriene B4 standard
Fish Leukotriene B4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Leukotriene B4 is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind to and cross it into the tissue. In neutrophils, it is also a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosome enzymes by these cells.
Leukotriene B4 is a potent pro-inflammatory molecule that belongs to a family of eicosanoid lipid mediators. It is synthesized from arachidonic acid that is generated from nuclear membrane phospholipid. In general, and upon cell activation, phospholipase A2 is directed to the nuclear membrane. Here, it generates arachidonic acid that is bound by one 23 kDa membrane-bound protein termed Five-Lipooxygenase Activating Protein (FLAP).