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Bovine Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit

Bovine Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit

Item no.	ABK-KTE10376-48T
Manufacturer	Abbkine Scientific
Amount	48T
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Bovine
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Applications notes	This Bovine Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP51A1 in samples. An antibody specific for CYP51A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP51A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP51A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP51A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
Sample type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	Bovine Lanosterol 14-alpha demethylase microplate Bovine Lanosterol 14-alpha demethylase standard Bovine Lanosterol 14-alpha demethylase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features & Benefits	Bovine Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine CYP51A1. No significant cross-reactivity or interference between Bovine CYP51A1 and analogues was observed.
Usage notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Lanosterol 14-alpha demethylase (CYP51A1) has a alternative name : Cytochrome P450 Family 51 Subfamily A Member 1, This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. This protein acts as an enzyme. It is known to catalyze the following reaction : A 14-alpha-methylsteroid + 3 O(2) + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP(+) + 4 H(2)O.
Gene ID	505060
Alternative	CYP51A1; CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1; cytochrome P450; 51 (lanosterol 14-alpha-demethylase); lanosterol 14-alpha-demethylase; sterol 14-alpha demethylase
Accession	Q4PJW3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 48T

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Delivery expected until 10/18/2024

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