Item no. |
ABK-KTE10322-48T |
Manufacturer |
Abbkine Scientific
|
Amount |
48T |
Category |
|
Type |
Elisa-Kit |
Applications |
ELISA |
Specific against |
Bovine |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Available |
|
Applications notes |
This Bovine Serine/threonine-protein kinase greatwall (MASTL) ELISA Kit employs a two-site sandwich ELISA to quantitate MASTL in samples. An antibody specific for MASTL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMASTL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASTL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASTL bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Bovine Serine/threonine-protein kinase greatwall microplate Bovine Serine/threonine-protein kinase greatwall standard Bovine Serine/threonine-protein kinase greatwall detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Bovine Serine/threonine-protein kinase greatwall (MASTL) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MASTL. No significant cross-reactivity or interference between Bovine MASTL and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Gandhi et al. (2003) identified MASTL, which they called FLJ14813, within the region of chromosome 10p where Drachman et al. (2000) had mapped autosomal dominant thrombocytopenia, also known as thrombocytopenia-2 (THC2), by linkage analysis. The protein product of the MASTL gene is a putative kinase that contains 2 highly conserved kinase domains. Gandhi et al. (2003) noted that a similar gene, 'greatwall, ' had been described in Drosophila. EMS-induced mutations in the greatwall gene cause a metaphase-arrest phenotype and problems in chromosome condensation.By extensive sequencing of multiple genes located in the critical segment on 10p, Gandhi et al. (2003) demonstrated a missense mutation in the MASTL gene . |
Alternative |
MASTL; FLJ14813; RP11-85G18.2; THC2; |
Accession |
E1BFR5 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.