Comparison

Bovine S-adenosylmethionine synthase isoform type-1 (MAT1A) ELISA Kit

Item no. ABK-KTE10321-96Tx50
Manufacturer Abbkine Scientific
Amount 96Tx50
Category
Type Elisa-Kit
Applications ELISA
Specific against Bovine
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Bovine S-adenosylmethionine synthase isoform type-1 (MAT1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAT1A in samples. An antibody specific for MAT1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAT1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAT1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAT1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Bovine S-adenosylmethionine synthase isoform type-1 microplate
Bovine S-adenosylmethionine synthase isoform type-1 standard
Bovine S-adenosylmethionine synthase isoform type-1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Bovine S-adenosylmethionine synthase isoform type-1 (MAT1A) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MAT1A. No significant cross-reactivity or interference between Bovine MAT1A and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
MAT1a catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency.The peptide maps of the 2 forms were possibly identical, although they showed different specific activities at physiologic concentrations of methionine. The findings suggested that the 2 isoforms are encoded by the same gene.
Gene ID
541078
Alternative
MAT1A; RP11-36D19.2; MAT; MATA1; SAMS; SAMS1; S-adenosylmethionine synthetase
Accession
Q2KJC6

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96Tx50
Available: In stock
available

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