Bovine Phosphomevalonate kinase (PMVK) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Bovine Phosphomevalonate kinase microplate
Bovine Phosphomevalonate kinase standard
Bovine Phosphomevalonate kinase detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

PMVK (EC 2.7.4.2) is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate as the fifth reaction of the cholesterol biosynthetic pathway. The deduced 192-amino acid PMVK protein has a calculated molecular mass of about 22 kD. It contains a C-terminal peroxisomal targeting sequence, and a single methionine is removed from the N terminus upon maturation of the protein. Expression was highest in heart and skeletal muscle, with slightly lower levels in liver, kidney, and pancreas, and low but detectable levels in brain, lung, and placenta.Analysis of transcripts from human lymphoblasts subcultured in lipid-depleted sera (LDS) and LDS supplement with lovastatin indicated that PMVK expression is subject to regulation by sterol at the level of transcription.

PMVK; HUMPMKI; PMK; PMKA; PMKASE;