Item no. |
ABK-KTE10137-96Tx50 |
Manufacturer |
Abbkine Scientific
|
Amount |
96Tx50 |
Category |
|
Type |
Elisa-Kit |
Applications |
ELISA |
Specific against |
Bovine |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Available |
|
Applications notes |
This Bovine Glutaminyl-tRNA synthetase (QARS) ELISA Kit employs a two-site sandwich ELISA to quantitate QARS in samples. An antibody specific for QARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QARS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QARS bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Bovine Glutaminyl-tRNA synthetase microplate Bovine Glutaminyl-tRNA synthetase standard Bovine Glutaminyl-tRNA synthetase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Bovine Glutaminyl-tRNA synthetase (QARS) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine QARS. No significant cross-reactivity or interference between Bovine QARS and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. |
Gene ID |
514586 |
Alternative |
QARS; GLNRS; PRO2195; glutamine tRNA ligase; glutamine-tRNA synthetase |
Accession |
Q3MHH4 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.