Comparison

IPKine HRP, Mouse Anti-Rabbit IgG LCS

Item no. ABK-A25022-500ul
Manufacturer Abbkine Scientific
Amount 500ul
Category
Type Antibody Monoclonal
Certificate HRP
Applications ELISA, Western Blot, Immunohistochemistry
Specific against Rabbit
Host Mouse
Isotype IgG
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Available
Immunogen
Rabbit IgG light chain
Applications notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:100000), IHC-P (1:500-1:5000), ELISA (1:5000-1:100000).
Purification
Affinity purified using solid phase Rabbit IgG(H+L) light chain with finally > 95% purity based on SDS-PAGE
Conjugate
HRP
Formulation
Liquid solution
Features & Benefits
50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). Abbkine's IPKine products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots).
Storage buffer
Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
Storage instructions
Stable for one year at -20C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.
Other info
The antibody reacts with kappa light chains on rabbit IgG, while it doesn’t react with the heavy chain of rabbit IgG. It's cross-reaction with human, mouse, rat, goat, sheep and bovine serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500ul
Available: In stock
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