Comparison

Dylight 488, Rabbit Anti-Goat IgG

Manufacturer Abbkine Scientific
Category
Type Antibody Polyclonal
Specific against Goat
Isotype IgG
Applications Immunofluorescence, Flow cytometry, Immunocytochemistry
Amount 500ul
Host Rabbit
Item no. ABK-A23230-500ul
eClass 6.1 32160702
eClass 9.0 32160702
Available
Immunogen
Goat IgG whole molecule
Applications notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.
Purification
Affinity purified using solid phase Goat IgG (H&L) with finally > 95% purity based on SDS-PAGE
Conjugate
Dylight 488
Formulation
Liquid solution
Features & Benefits
DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).
Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Storage instructions
Stable for one year at -20C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
Other info
The antibody reacts with whole molecule goat IgG. It also reacts with heavy chains of goat IgG, and light chains of all other goat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500ul
Available: In stock
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