BL21 Chemically Competent Cell is designed for chemical transformation of DNA. This cell is resistant to tetracycline (Tet
R), with a transformation efficiency of over 10
7 cfu/µg DNA (tested by pUC19 plasmid DNA). The control plasmid (Amp
+) is used for detection of cell expression functions (protein size: ~25 kDa). Tight expression control is particularly suitable for toxic protein expression.
The genotype is:
E. coli B F
- dcm omp T
hsdS(r
B-m
B-)
gal [
malB
+]
K-12(λ
S).
The 1 ml size consists of 10 × 100 µl Competent Cells, and 20 μl (0.1 ng/μl) Control Plasmid pUC19.