DE3 Chemically Competent Cell (KanR, TetR)

DE3 Chemically Competent Cell is designed for chemical transformation of DNA, with a transformation efficiency of over 10⁷ cfu/µg DNA (tested by pUC19 plasmid DNA). This cell is resistant to kanamycin (Kan^R) and tetracycline (Tet^R), and thioredoxin reductase (trxB) and glutathione reductase (gor) mutations greatly facilitates cytoplasmic disulfide bond formation. The control plasmid (Amp⁺) is used for detection of cell expression functions (protein size: ~25 kDa).

The genotype is: F^- ompT hsdSB(r_B^-m_B^-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (Kan^R, Tet^R).

The 1 ml size consists of 10 × 100 µl Competent Cells, and 20 μl (0.1 ng/μl) Control Plasmid pUC19.

Contains ≤ 10% glycerol.

Store at -70 °C for up to 6 months. Do not store in liquid nitrogen. Avoid repeated freeze/thaw cycles.