Blue Chemically Competent Cell is designed for chemical transformation of DNA. This cell is resistant to tetracycline (Tet
R) and chloramphenicol (Cam
R), and allows blue/white selection with a transformation efficiency of over 10
8 cfu/µg DNA (tested by pUC19 plasmid DNA). It is suitable for larger plasmids, recombinant products, and library construction.
The genotype is: Tet
RΔ (
mcrA)183Hte[F'{
proAB
lacI
q lacZΔ M15
Tn10(Tet
R)AmyCam
R}]Δ (
mcrCB-
hsdSMR-
mrr)173
endA1
supE44
thi-1
recA1
gyrA96
relA1.
The 1 ml size consists of 10 × 100 µl Competent Cells, 20 μl (0.1 ng/μl) Control Plasmid pUC19, and 10 ml SOC Medium.