Chemically Competent Cell is designed for chemical transformation of DNA, with a transformation efficiency of over 10
8 cfu/µg DNA (tested by pUC19 plasmid DNA). It is suitable for blue/white selection, routine cloning, and is the lowest homologous recombination favourable for plasmid DNA preparation.
The genotype is:
endA1
recA1
gyrA96
thi-1
hsdR17 (r
k-, m
k+)
relA1
supE44 D (
lac-proAB) [F'
traD36
proAB
laqI
qZΔ M15].
The 1 ml size consists of 10 × 100 µl Competent Cells, 20 μl (0.1 ng/μl) Control Plasmid pUC19, and 10 ml SOC Medium.