hTERT Antibody and Peptide Kit

The hTERT antibody is a sheep anti-human polyclonal which provides superior telomerase immunoprecipitation. This kit contains a solution of antigenic release peptide to facilitate dissociation of the hTERT antibody resulting in elution of telomerase after immunoprecipitation. The kit includes 100 µg of antibody and 125 µl of peptide.For more information please see the Nature Communications publication: Moye, A., Porter, K., Cohen, S. et al. Telomeric G-quadruplexes are a substrate and site of localization for human telomerase. Nat Commun 6, 7643 (2015). The working concentration for the antibody is approximately 20 µg of antibody per ml of lysate and for the peptide 25 µl peptide per 20 µg antibody (corresponding to 20 molar equivalents of peptide per antibody).

Notes: Based on immunoprecipitation of telomerase from HEK-293 tumour cell lysates, followed by a 1 hr elution in the presence of antigenic peptide, if available. There are no existing peptide antigens for the Calbiochem, Novus, Abcam, Rockland or Epitomics antibodies. Background activity in control represents non-specific adhesion to Protein G-Agarose beads.

Antibody: 10 mM HEPES-KOH (pH 8), 100 mM KCl, 0.05 w/v NaN3, 10% v/v glycerol. Peptide: H2O with 10% v/v glycerol + 0.05% w/v NaN3.

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hTERT Antibody and Peptide Kit