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PARP1 Antibody

PARP1 Antibody

ArtNr	PRS-13-003-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Neuroscience Molecular Targets for Neuroscience Immunohistochemistry Cell Lines & Cell Culture Tissues
Typ	Slides
Format	Liquid
Applikationen	WB, IF
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160905
ECLASS 11.0	32160905
UNSPSC	41121800
Alias	KIF3B, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1
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Shipping	blue ice or RT
By Research Area	Cell Cycle, Immunology, Signal Transduction
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2).
Applications	WB: 1:1000 - 1:2000 IF: 1:50 - 1:200
Positive Control 1	Jurkat
Positive Control 2	HeLa
Positive Control 3	RAW264.7
Positive Control 4	C6
Positive Control 5	Mouse testis
Positive Control 6	Mouse brain
Predicted Molecular Weight	Observed: 116kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PARP1
Ncbi Gene Id #	142
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PARP1
Ncbi Official Full Name	poly (ADP-ribose) polymerase 1
Ncbi Organism	Homo sapiens
Swissprot #	P09874
Background	This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
2nd Image Caption	Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
3rd Image Caption	Immunofluorescence analysis of NIH/3T3 cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining.
4th Image Caption	Immunofluorescence analysis of U2OS cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 uL

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Lieferung vsl. bis 04.10.2024

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