ArtNr |
PRS-13-003-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Slides |
Format |
Liquid |
Applikationen |
WB, IF |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160905 |
ECLASS 11.0 |
32160905 |
UNSPSC |
41121800 |
Alias |
KIF3B, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Immunology, Signal Transduction |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2). |
Applications |
WB: 1:1000 - 1:2000
IF: 1:50 - 1:200 |
Positive Control 1 |
Jurkat |
Positive Control 2 |
HeLa |
Positive Control 3 |
RAW264.7 |
Positive Control 4 |
C6 |
Positive Control 5 |
Mouse testis |
Positive Control 6 |
Mouse brain |
Predicted Molecular Weight |
Observed: 116kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PARP1 |
Ncbi Gene Id # |
142 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PARP1 |
Ncbi Official Full Name |
poly (ADP-ribose) polymerase 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P09874 |
Background |
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
2nd Image Caption |
Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
3rd Image Caption |
Immunofluorescence analysis of NIH/3T3 cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining. |
4th Image Caption |
Immunofluorescence analysis of U2OS cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.