Anti-Ki67 antibody

Unconjugated

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Store at-20° C, and avoid repeat freeze-thaw cycles.

Ki67 is a protein encoded by the MKI67 gene which is approximately 358, 6 kDa. Ki67 is localised to the nucleus. It is involved in DNA damage and neuroscience. It is a nuclear protein that is associated with and may be necessary for cellular proliferation. It is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. It also associates with the surface of the mitotic chromosome, the peri-chromosomal layer, and covers a substantial fraction of the chromosome surface. Ki67 is expressed preferentially during late G1, S, G2 and M phases of the cell cycle. Mutations in the MKI67 gene may result in brain ependymoma and amyloplastic carcinoma. STJ93832 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Ki-67 protein.

MKI67

3170-3250 aa, C-terminal

Chromosome
Nucleus
Nucleolus
Associates With The Surface Of The Mitotic Chromosome
The Perichromosomal Layer
And Covers A Substantial Fraction Of The Mitotic Chromosome Surface
Associates With Satellite Dna In G1 Phase
Binds Tightly To Chromatin In Interphase
Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes
Predominantly Localized In The G1 Phase In The Perinucleolar Region
In The Later Phases It Is Also Detected Throughout The Nuclear Interior
Being Predominantly Localized In The Nuclear Matrix

IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Proliferation Marker Protein Ki-67
Antigen Identified By Monoclonal Antibody Ki-67
Antigen Ki-67
Antigen Ki67

KI67_HUMAN